How HPLC works
A dissolved sample is pumped at high pressure through a column packed with fine particles. Different components travel through at different speeds, so they come out at different times.
A detector records each component as a peak on a graph called a chromatogram. For peptides the detector usually reads ultraviolet light at around 214 to 220 nm, where peptide bonds absorb.
How the percentage is worked out
Purity is the area of the main peak divided by the combined area of every peak, times 100. If the main peak is 99 units of area out of 100, the purity is 99%.
What HPLC purity does not tell you
Educational information only. Nothing here is medical advice, dosing guidance or a recommendation to use any substance. Research peptides are not approved medicines and are not for human or animal consumption. Speak to a qualified professional and follow the law where you are.
