Weighing molecules
A mass spectrometer gives molecules an electric charge, then measures how they move in electric or magnetic fields. The result is the mass-to-charge ratio, written m/z.
Peptides are usually ionised by electrospray (ESI) or MALDI. Larger peptides often pick up more than one charge, so a single compound can show several peaks.
Reading the result
Compare the measured mass with the expected molecular weight. For BPC-157 (1,419.5 g/mol) you might see [M+H]+ near 1,420.5 or [M+2H]2+ near 710.8. A mass far from the expected value means the vial holds something else.
Why it is paired with HPLC
Mass spectrometry answers "is this the right compound?" HPLC answers "how pure is it?" A trustworthy certificate shows both, for the same batch.
Educational information only. Nothing here is medical advice, dosing guidance or a recommendation to use any substance. Research peptides are not approved medicines and are not for human or animal consumption. Speak to a qualified professional and follow the law where you are.
